Protein A Affinity Resin A50
Biovanix protein A50 resin is designed to analyse and purify monoclonal antibodies (mAbs). Compared to traditional agarose media, Biovanix protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).
The ligand of Biovanix protein A50 is recombinant protein A (rProtein A) immobilised on the surface of the macro-porous PS/DVB microsphere substrate. The rProtein A has a better alkali-resisting ability that ensures stability in high pH conditions. With our hydrophilic treatment and binding technology, we eliminated non-specific binding PS-DVB surface. Hence, Biovanix protein A50 is extremely useful for the purification process of monoclonal antibodies.
Advantages：
- High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing
- Outstanding high dynamic binding capacity at low residence time reduces process time and the amount of medium used
- Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP
- Low ligand leakage ensures a long lifetime
- High price-performance ratio
Main Characteristics
| Support Matrix | Poly(styrene/divinylbenzene) (PS-DVB) |
| Ligand | Recombinant Protein A |
| Ave. Particle size | 50µm |
| Dynamic Binding Capacity (DBC) | Approx. 40 mg human IgG/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 5 cm, Residence time 0.6 min) |
| Shrinkage/Swelling | < 1% from 1-100% organic solvent |
| pH range (Long term) | pH 2-10 |
| Maximum Operating Pressure | 1500 psi (100 bar / 10 MPa) |
| Cleaning Agents | 0.1-0.5M NaOH |
| Temperature Stability | 4-40 °C |
| Delivery Conditions | 20% ethanol (2-8℃) |
Performance Testing
1. Particle images
2. Dynamic Binding Capacity (DBC)
| Test Condition | |
|---|---|
| System | AKTA® Purifier10 |
| Initial Buffer | 20 mM PB，0.15 M NaCl，pH 7.4 |
| Elution Buffer | 0.1 M Gly-HCl, pH 2.5 |
| CIP solution | 0.5 M NaOH |
| Sample | 1.0 mg/ml hIgG |
3. Protein A Ligand Leakage Test (ELISA)
Samples: 20mg hlgG/ml-resin, 9mg mAb/ml-resin
ELISA test: Cygnus F400 kit
4. Alkali Resistance Test (150 Cycle Lifetime)
Related Products
Agarose Substrate
- High cross-linked agarose
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
Ni Affinity Resin
- Ni NTA/NTA+ Resin
- Ni IDA Resin
- Ni TED Resin
- Benzamidine Affinity Media
mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range
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