Perfusion Chromatography Resin
Combining innovative bonding and surface treatment techniques, Biovanix developed a series of high-performance perfusion chromatography resins. It is a hydrophilic porous polymer microsphere developed based on PS-DVB microspheres with extremely low non-specific adsorption. The media are top-performing chromatographic media for process-scale bioseparations. The rigid, robust particles enable high-resolution separations with 2–3x the throughput of conventional fast-flow gels. They are easier to handle and pack, and offer outstanding cleanability.
Perfusion chromatography media offer better choices for your purification processes in biological science, biological engineering, and the pharmaceutical industry.
Mutiple Choices
Ion-exchange, affinity and hydrophobic interactive media. Multiple choices for different applications.
Rigid Microsphere With Large Pore Size
The core of perfusion chromatography particles is the novel porous structure with large “throughpores”. These large throughpores allow part of the mobile phase to flow through, quickly carrying biomolecules to smaller diffusive pores. The large “throuhpores” reduce the diffusion rate of biomolecules and enhance interaction between biomolecules and functional groups on the surface. Consequently, diffusion barriers are lowered, and flow rate can be dramatically increased-without compromising capacity or resolution.
- 3000-6000Å pore size, enables the diffusion and mass transfer for large biomolecules.
- 45-90 micron particles satisfy your purification processing requests.
Flexible Tentacles
Flexible tentacle structure minimises the steric hindrance between functional groups and target molecules. It also improves the binding capability of the target material.
Compared to traditional media, perfusion chromatography media have more effective capture and higher total recovery.
High Flow Rate, Low Backpressure
VirCap® media offer an excellent balance of resolution and operating backpressure.
Under the condition of mobile phase change, VirCap® media exhibites almost no shrinkage or expansion. Throughpores and flexible tentacles ensures the rapid diffusion of solute, reduces the barrier of mass transfer, and realizes the high dynamic load under the operation of high flow rate.
Robust Chemical Stability
VirCap® media are rigid polymeric particles coated with a proprietary hydrophilic polymer onto which the various functional groups (ion exchange, affinity, etc.) are covalently attached. The result is a highly robust, chemically stable product that is ideally suited for large-scale biopharmaceutical applications.
Clean-in-Place Condition (CIP)
0.5-1M NaOH, organic solvent, high salt solvent.
Prosep AF Perfusion Chromatography Resin
Virus purification is the basis of developing high-quality vaccines, and also provides an important basis for the study of virus fine morphological structure, isolation and purification of virus antigen protein, and detailed studies of virus chemical composition and genetic material.
In traditional virus vaccine purification processing, the sucrose density gradient ultrafast purification process requires high-cost, large centrifugal devices. These devices with small sample loading, long treatment time, and poor removal of host cell DNA. Gel filtration chromatography, widely used in a vaccine purification process, is mild, repeatable and easy to industrialise. But its efficiency is low, and the effect of removing heteroprotein and DNA is not obvious.
Prosep AF media is an affinity chromatography media designed for the capture and moderate purification stages of capsular virus purification. Specific adsorption of prosep AF media and target occurs by simulating the affinity between ligands and virus particles with capsular membranes. With unique high loading capacity, high flow rate and low back pressure, Prosep AF media reduces the process cycle time and increases the yield, fully meeting the requirements of large-scale biopharmaceutical and vaccine production processes.
Prosep AF Media Advantages:
√ High selectivity, similar affinity to live and inactivated capsular viruses.
√ Closed operation, safety, and sterility.
√ Enrichment and purification are carried out simultaneously, increasing production, reducing processing steps, time and cost.
√ Rigid particles can be operated under a high flow rate, which improves the purification efficiency.
√ Good chemical stability, longer service life.
Prosep AF Resin Parameter
| Substrate | Hydrophilic PS/DVB Microspheres |
| Particle Size | 70um |
| Dynamic Binding Capability | 100mg lysozyme/ml |
| Flow Rate | 1000cm/h
(20℃, buffer solution viscosity same as water，pressure < 3 bar / 43.5psi, column bed height 20cm) |
| Column Bed Height | 20-40cm |
| pH Stability | 1-14 |
| Working Temperature | 4-30℃ |
| CIP Condition | 0.5-1M NaOH |
| Storage | 2-8℃ 20% EtOH |
Prosep AF Resin Application
| Viruses | Viral/Microbial Antigens | |
| Rabies | Feline Calicivirus | Herpes Simplex gA and gB Glycoprotein Subunits |
| Influenza | Respiratory Syncytial Virus | Hepatitis B Surface Antigen |
| Japanese Enchephalitis | Human Herpes Simplex | Filamentous Hemagglutinin from B. pertussis |
| Feline Leukemia | Human Measles | Leucocytosis Promoting Factor Hemagglutinin |
| Feline Herpes | Human Parainfluenza | |
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