Packing Materials For HPLC Columns
Biovanix packing material for reversed chromatography uses high-purity porous silica microspheres made of high-purity organic silicon. Total metal ion content < 20 ppm. Mainly used to separate peptides, proteins, and other biomacromolecules.
Silica, C18, C8, NH2, CN, and phenyl columns are available.
- Uniform particle size, High column efficiency, High resolution
- Low poriness, no fragments in repeated packing
- Excellent C18/C8 bonding and end-capping technologies
- Multiple choice for different requests
- Customisation is available.
Material Characters
Reversed-phase Chromatography
| Products | Particle Size | Pore Size | Surface Area | Carbon Content | pH Range | End-caped |
| C18 | 3/5/10 um | 100Å | 330m2/g | 16% | 2-8 | Yes |
| C18H | 5/10 um | 120Å | 330m2/g | 20% | 2-11 | Yes |
| C18L | 5/10 um | 120Å | 330m2/g | 13% | 2-8 | Yes |
| C8 | 5/10 um | 120Å | 330m2/g | 12% | 2-8 | Yes |
| C4 Bio | 5/10 um | 300Å | 100m2/g | 3% | 2-8 | Yes |
| C8 Bio | 5/10 um | 300Å | 100m2/g | 5% | 2-8 | Yes |
| C18 Bio | 5/10 um | 300Å | 100m2/g | 8% | 2-8 | Yes |
| Phenyl | 5/10 um | 120Å | 330m2/g | 8% | 2-8 | Yes |
| Products | Particle Size | Pore Size | Surface Area | Carbon
Content |
pH Range | End-caped |
| C18 | 15/20/30/50 um | 120Å | 330m2/g | 16% | 2-8 | Yes |
| C8 | 20/30/50/75 um | 120Å | 330m2/g | 12% | 2-8 | Yes |
Normal-phase Chromatography
| Products | Particle Size | Pore Size | Surface Area | Carbon Content | pH Range | End-capped |
| SiO2 | 3/5/8/10 um | 120Å | 330m2/g | - | 2-8 | - |
| SiO2 Bio | 5/10 um | 300Å | 330m2/g | - | 2-8 | - |
| NH2 | 5/10 um | 120Å | 330m2/g | 5% | 2-8 | Yes |
| CN | 5/10 um | 120Å | 330m2/g | 7% | 2-8 | Yes |
| Diol | 5/10 um | 120Å | 330m2/g | 8% | 2-8 | Yes |
| Products | Particle Size | Pore Size | Surface
Area |
Carbon
Content |
pH Range | End-capped |
| SiO2 | 15/20/30/50 um | 120Å | 330m2/g | - | 2-8 | - |
The parameters will based on the certification of analysis attached to products.
C18/C8 Performance And Application Tests
1. Particle size distribution test and electron microscope photos
- Uniform particle size distribution.
- Pores on surface distribute evenly.
2. NST (National Institute of Standards and Technology) SRM870 for C18 packing material
- Low metal content
- Very low activity of the silicon hydroxyl group
- Perfect end cap technology
- Uniform bonding
The symmetry As of peak 4 (quinone akane) and peak 5 (amittiline) in the figure respectively characterized the content of metal impurities and the activity of residual silicon hydroxyl groups in the column. The degree of separation of peak 2 (tol) and peak 3 (ethylbenzene) characterises the degree of bonding uniformity.
According to the results, the quinone akane peak type symmetry of C18 indicates that the content of metal impurities in the filler is very low. The excellent amittiline peak shape indicates that the filler can be used for the separation of alkaline compounds. The perfect separation indicates the bond is uniform and can separate compounds with only one difference of methylene.
3. Repacking Test For 20 Circles
- The percentage of particle breakage is low.
- One batch C18/C8 packing material can be repacked for over 20 circles with proper usage.
Testing column: 4.6mm*250mm column.
Follow the standard packing method for C18/C8 packing materials.
Retest the C18 column after the packing process.
Application
Tips:
- Chemical compounds in packing material slurry affect the peak shape and trail.
- Clean the column after purification of the sticky samples.
- Operate carefully when packing the C18 packing material.
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