Ion-exchange Chromatography Resins
Ion-exchange chromatography (IEX) is one of the most widely used chromatography techniques, which has played an important role in the separation and purification of biomolecules since the 1960s. IEC controls the reversible interaction between charged molecules and ion exchange media with opposite charges to achieve the binding and elution of specific molecules, to achieve the separation effect. IEC is widely used in protein, skin, nucleic acids and other electric charges in the separation and purification of biomolecules. The advantages of high capacity and high resolution to well thousand among the whole process of capture, purification, and fine purification stage. IEC applies to the analysis of a small amount of sample, and the purification of sample preparation.
Biovanix Ion-exchange Chromatography resins use agarose and monodisperse PS-DVB microspheres for substrates. For PS-DVB microspheres substrate, it has the advantages of high mechanical strength and excellent pressure resistance to meet the high requirements of preparative production conditions.
Technology Advantages
Biovanix ion-exchange packing materials are a series of ion exchange media developed based on monodisperse PSDVB microspheres. Its matrix structure and hydrophilic polysaccharide base material, such as agarose, cellulose, is completely different. First of all, it has excellent mechanical properties, can withstand up to 10MPa pressure; Secondly, the large pore size of 1000A can guarantee the free entry and exit of biological macromolecules. Finally, the special coating technology of Biovanix gives it enough hydrophilicity to ensure its excellent biocompatibility. The monodisperse particle size can effectively reduce the column pressure and mass transfer obstruction.
Biovanix ion-exchange packing materials use "tentacles" surface-derived technology, functional groups in the form of a linear polymer chain covalently combined on the surface of the substrate. Relative to the traditional ion exchange medium directly on the surface modification or with only short-chain in combination with the pattern of functional groups, Biovanix ion-exchange packing materials has more effective number of functional groups, not only its space steric hindrance to reduce macromolecular protein, antibody, viruses, and plasmid can more effectively combine with medium of functional groups, significantly increase capacity, and "tentacles" structure can effectively reduce enough biological molecules and nonspecific interaction between the dielectric substrate, so as to improve the recovery rate of target molecules.
AstraChroma PSDVB Resin
| Product | Poly15 SP | Poly15 Q | Poly30 SP | Poly30 Q |
| Matrix | Monodisperse PS-DVB | |||
| Particle Size | 15um | 30um | ||
| Function Group | (-CH2)SO3- | -CH2N+(CH3)3 | (-CH2)SO3- | -CH2N+(CH3)3 |
| Ligand Density | 0.22meq/mL | 0.24meq/mL | 0.15meq/mL | 0.18meq/mL |
| Capacity | 80mg Lys/mL | 45mg BSA/mL | 60mg Lys/mL | 30mg BSA/mL |
| Flow Rate | 150~800cm/h | 250~1000cm/h | ||
| Max. Pressure | 8.0MPa | 5.0MPa | ||
| pH Stability | 2-12 | |||
| Chemical Stability | All commonly used buffers,1M acetic acid,1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea, and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
| Usage Temperature | 4~30°C | |||
| Storage | 2~30°C, 20% ethanol | |||
| Product | Poly 50M | |||
| Matrix | SP | Q | CM | DEAE |
| Particle Size | PS-DVB | |||
| Function Group | 50um | |||
| Pore Size | 100-150nm | |||
| Ligand Density | 0.15meq/mL | 0.16meq/mL | 0.15me q/mL | 0.16me q/mL |
| Capacity | >80mgLys | >100mg BSA | >80mgLys | >90mg BSA |
| Flow Rate | 300~1200cm/h | |||
| Max Pressure | 3.0MPa | |||
| pH Stability | 1-12 | |||
| Chemical Stability | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
| Usage Temperature | 4~30°C | |||
| Storage | 2~30°C, 20% ethanol | |||
| Product | Poly 50G | |||
| Matrix | SP | SP | SP | SP |
| Particle Size | PS-DVB | |||
| Function Group | 50um | |||
| Pore Size | 150-300nm | |||
| Ligand Density | 0.14me q/mL | 0.14me q/mL | 0.14me q/mL | 0.14me q/mL |
| Capacity | >70mg Lys | >70mg Lys | >70mg Lys | >70mg Lys |
| Flow Rate | 300~1200cm/h | |||
| Max Pressure | 2.0MPa | |||
| pH Stability | 1-12 | |||
| Chemical Stability | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
| Usage Temperature | 4~30°C | |||
| Storage | 2~30°C, 20% ethanol | |||
| Product | Poly 50V | |||
| Matrix | SP | SP | SP | SP |
| Particle Size | PS-DVB | |||
| Function Group | 50um | |||
| Pore Size | 300-400nm | |||
| Ligand Density | 0.12me q/mL | 0.12me q/mL | 0.12me q/mL | 0.12me q/mL |
| Capacity | >70mg Lys | >70mg Lys | >70mg Lys | >70mg Lys |
| Flow Rate | 300~1200cm/h | |||
| Max Pressure | 1.0MPa | |||
| pH Stability | 1-12 | |||
| Chemical Stability | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
| Usage Temperature | 4~30°C | |||
| Storage | 2~30°C, 20% ethanol | |||
Ion-exchange Chromatography Media
| Product | Dynamic Binding Capacity | Application |
| DEAE 6FF | 60 mg BSA/mL | Weak anion exchange medium: High Applicability (FF) High Resolution (HP) High Capacity (XL) |
| DEAE 6HP | 60 mg BSA/mL | |
| DEAE 6XL | 120 mg BSA/mL | |
| Q 6FF | 50 mg BSA/mL | Strong anion exchange media: High Applicability (FF) High Resolution (HP) High Capacity (XL) |
| Q 6HP | 70 mg BSA/mL | |
| Q 6XL | 140 mg BSA/mL | |
| CM 6FF | 100 mg LZM /mL | Weak cation exchange medium: High Applicability (FF) High Resolution (HP) High Capacity (XL) |
| CM 6HP | 120 mg LZM /mL | |
| CM 6XL | 120 mg LZM /mL | |
| SP 6FF | 130 mg LZM /mL | Strong cation exchange medium: High Applicability (FF) High Resolution (HP) High Capacity (XL) |
| SP 6HP | 160 mg LZM /mL | |
| SP 6XL | 160 mg LZM /mL |
Biovanix Prosep series is based on the Cytiva Capto series. It is a bioseparation medium developed for near-rigid cross-linked agarose microspheres. Prosep has nearly rigid physical properties, narrower microsphere distribution, more reasonable average particle size, and more protein adsorption space, which reflects higher adsorption capacity, lower chromatographic back pressure, higher operating flow rate and higher resolution in the chromatography process, and is a new generation of high-performance and cost-effective chromatography media. The ion exchange medium based on Prosep matrix has excellent performance and is widely used in laboratory scale preparation of biological macromolecules such as proteins, nucleic acids, peptides and polysaccharides, and large-scale industrial preparation of biopharmaceuticals and bioengineering.
Advantages:
• Faster operating flow rate
• Faster mass transfer
• Higher dynamic load
• Higher resolution
• Higher voltage resistance
• Low operating pressure
| Product | Dynamic Binding Capacity | Application |
| Prosep DEAE | 90 mg BSA/mL | High rigidity
High flow rate High resolution Quick loading |
| Prosep Q | 120 mg BSA/mL | |
| Prosep SP | 120 mg lysozyme/mL | |
| Prosep DEAE HPR | 35 mg BSA/mL | |
| Prosep Q HPR | 45 mg BSA/mL | |
| Prosep CM HPR | 75 mg lysozyme/mL | |
| Prosep SP HPR | 70 mg lysozyme/mL |
Selection Of Ion-exchange Packing Materials
In practical applications, the substrate type, pore structure, particle size and distribution, substrate type, and density of ion-exchange media will affect the tomographic effect of the medium.
- The porosity of the substrate provides a large surface area covered with charged groups, thus ensuring a high bonding capacity. However, the large pore size ensures the effective mass transfer of protein biomolecules.
- The hydrophilic substrate has better biocompatibility and less non-specific adsorption.
- The high chemical stability ensures that the medium can be cleaned with a vigorous cleaning solution if necessary.
- The high physical stability ensures that the volume of the column bed. It remains constant when the concentration of salt ions or the pH value changes dramatically. This can increase the repeatability of the system, less unnecessary reloading columns. At the same time, the consistency of particle size distribution is beneficial to the operation of high-speed liquid flow, especially in cleaning or rebalancing steps, which can increase the flux and working capacity of the system.
| Cation-exchange | ||
| Sulfonic Group S/SP | Strong Type | -SO32- |
| Carboxymethyl CM | Week Type | -COO |
| Anion-exchange | ||
| Quaternary Ammonium Q | Strong Type | -N+(CH3)3 |
| Tertiary Amine D | Week Type | -N+H(CH3)2 |
Strong ion-exchange media have the following advantages
- Since the charged nature of the medium does not change with the change of pH, the establishment and optimization of the separation process will be very fast and easy.
- Since there is no intermediate form of charge interaction, the interaction mechanism is simple.
- At high or low pH, the binding capacity of the sample is maintained because the ion-exchange medium does not lose charge.
Most proteins have isoelectric points between 5.5 and 7.5 and can be separated by a strong or weak ion exchange medium. The advantage of weak ion-exchange media, such as D and CM, is that they offer different selectivity than strong ion-exchange media. The disadvantage is that weak ion-exchange media gain or loses protons as pH changes, so their ion-exchange capacity changes as pH changes.
When the desired selectivity cannot be obtained with strong ion-exchange media (Q, S, SP), weak ion-exchange media such as D, CM can be tried. When using a weak ion exchanger, use a float that minimizes the effect in the following pH range:
- D: pH 2-9
- CM: pH 6-10
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